Table 3. Relationship between S100A8 or S100A9 expression and clinicopathological parameters of patients.
| S100A8 expression | S100A9 expression | ||||||
| Negative | Positive | Negative | Positive | ||||
| ScoreI | ScoreII, III and IV | ScoreI | ScoreII, III and IV | ||||
| Characteristics | n | n (%) | n (%) | *p-Value | n (%) | n (%) | *p-Value |
| Gender | |||||||
| Male | 19 | 10 (52.6) | 9 (47.4) | p = 0.382a | 7 (36.8) | 12 (63.2) | p = 0.453a |
| Female | 23 | 9 (39.1) | 14 (60.9) | 6 (26.1) | 17 (73.9) | ||
| Age (years) | |||||||
| ≤65 | 21 | 12 (57.1) | 9 (42.9) | p = 0.121a | 8 (38.1) | 13 (61.9) | p = 0.317a |
| >65 | 21 | 7 (33.3) | 14 (66.7) | 5 (23.8) | 16 (76.2) | ||
| Location | |||||||
| Colon | 16 | 6 (37.5) | 10 (62.5) | p = 0.429a | 6 (37.5) | 10 (62.5) | p = 0.707a |
| Rectum | 26 | 13 (50) | 13 (50) | 7 (26.9) | 19 (73.1) | ||
| Tumor size (cm) | |||||||
| <5 | 23 | 13 (56.5) | 10 (43.5) | p = 0.106a | 10 (43.4) | 13 (56.6) | p = 0.053a |
| ≥5 | 19 | 6 (31.6) | 13 (68.4) | 3 (15.8) | 16 (84.2) | ||
| Histological differentiation | |||||||
| Well/moderately | 32 | 18 (56.3) | 14 (43.7) | p = 0.028b | 13 (40.6) | 19 (59.4) | p = 0.042b |
| Poorly | 10 | 1 (10) | 9 (90) | 0 (0) | 10 (100) | ||
| Dukes stage | |||||||
| A/B | 15 | 11 (73.3) | 4 (26.7) | p = 0.006a | 10 (66.7) | 5 (33.3) | p = 0.001a |
| C/D | ?? | 8 (29.6) | 19 (70.4) | 3 (11.1) | 24 (88.9) | ||
| Lymph node metastasis | |||||||
| Negative | 17 | 12 (70.6) | 5 (29.4) | p = 0.006a | 10 (58.9) | 7(41.1) | p = 0.001a |
| Positive | 25 | 7 (28) | 18 (72) | 3 (12) | 22 (88) | ||
CRC, colorectal carcinoma samples; normal tissues, matching distal normal tissues; n, number of samples. The proportion of staining of tumor cells was classified in four grades (<10% = 1, 10–25% = 2, 26–75% = 3, >75% = 4). In addition, its staining intensity was also divided into four subgroups (no staining = 0, weak = 1, moderate = 2, strong = 3). Staining index (value range, 0–12) = proportion×intensity. The expression of S100A8 and S100A9 was finally analyzed by a simplified score (scoreI, staining index 0–1; scoreII, staining index 2–4; score III, staining index 6–8; score IV, staining index 9–12). The expression of S100A8 and S100A9 in tumors with staining score of at least II were regards as positive. a, Pearson chi-squared test; b, Yates-corrected chi-squared test. *p-value<0.05 was considered significant.