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TS was done by DAPI (blue)/antibodies against CD146 (FITC/green) (for EC) and S100B (for brain) (rhodamine/red) (A–D: cBMECs, CD146+/S100B+/DAPI+). Cells indicated with arrows are cBMECs (A–E) from mice treated with PBS (A: Control), NT (B), METH (C), gp120 (D), and meningitic E. coli K1 E44 (E).
