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. 2013 Apr 26;8(4):e62164. doi: 10.1371/journal.pone.0062164

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© 2013 Huang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Triple staining (TS) of murine cBMECs and EPCs was done by DAPI (blue)/antibodies against CD146 (FITC/green) (for EC) and S100B (for brain) (rhodamine/red) (cBMECs, CD146+/S100B+/DAPI+) or CD133 (for PC/rhodamine/red) (EPCs, CD146+/CD133+/DAPI+). cBMECs and EPCs were counted with six random fields. Number of total ECs (CEC) (A), cBMECs (B) and EPCs (C) in peripheral blood (ml). Quantification of albumin in CSF (D). (**P<0.001).