Figure 4. Enhancement of cBMEC shedding in vitro by nicotine (NT), METH (MT) and gp120.

A. Shedding of cBMECs from BMEC monolayers in the upper chambers of Transwells after exposure to nicotine (10 µM), METH (10 nM) and gp120 (50 ng/ml) for 36 h. B. cBMEC shedding from the WT BMEC monolayers after exposure to different doses of nicotine. C. cBMEC shedding from WT (white column) and KO (black column) BMEC monolayers treated with METH. Bars denote mean values, and error bars describe SEM. ^* p<0.0 5; **P<0.01.