Figure 2.
Effect of S100A7 overexpression on invasiveness of MDA-MB-468 cells transfected with recombinants in Matrigel invasion assay. Cells were stably transfected with empty vector pc.DNA3.1 or pc.DNA3.1-S100A7, or transfected with S100A7 siRNA. After 2 days, 1 × 105 cells were allowed to invade through transwell inserts (8 μm) coated with Matrigel. The cells on the lower surface of the chambers were stained, counted, and photographed under a light microscope. Histogram shows invasive capability of transfected cells. Each bar represents mean ± SE (n = 3); *, P <0.05.