Figure 4.

S100A7 overexpression induced NF-κB in the MDA-MB-468 cells. A, NF-κB p65 expression in investigated MDA-MB-468 cells was analyzed by western blot. B, EMSA analysis was done for MDA-MB-468 cells. Nuclear extracts were prepared from control and transfected cells and subjected to analysis for NF-κB DNA-binding activity as measured by EMSA. Retinoblastoma protein level served as the nuclear protein loading control. EMSA, electrophoretic mobility shift assay; NF-κB, nuclear factor-kappa B.