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(A) pGreen lentiviral vector containing the HI PolII promoter driving the expression of multiple complementary bulged microRNA binding sites. Each binding site is complementary to a single microRNA with the exception of four mismatchs that create a bulge to promote sponge stability. [37] Separate promoters drive GFP and puromycin selection markers. (B) Table listing the individual miRs targeted by the various microRNA sponge constructs.
