Table 2.
Culture time, population doublings (pds), and growth rate of chondrocytic subpopulation after MACS separation
|
W5C5 sorting | |||||||||
|---|---|---|---|---|---|---|---|---|---|
|
Cell type / |
Mean |
no. of |
Mean |
Population doublings (pds) /day |
|||||
|
medium |
culture time |
passages |
pds |
Unsorted |
W5C5+ |
W5C5- |
|||
| [d] | Mean | sd | Mean | sd | Mean | sd | |||
| chondrocytes |
63.50 |
4 - 6 |
9.47 |
0.16 |
0.04 |
0.15 |
0.04 |
0.16 |
0.04 |
| n = 4 |
|
|
|
|
|
|
|
|
|
| chondrocytes |
24.50 |
4 |
11.53 |
0.51 |
0.07 |
0.49 |
0.05 |
0.48 |
0.04 |
| MSCGM; n = 2 |
|
|
|
|
|
|
|
|
|
| disc cells |
25.67 |
4 |
11.44 |
0.42 |
0.13 |
0.47 |
0.12 |
0.47 |
0.14 |
| n = 3 |
|
|
|
|
|
|
|
|
|
|
W8B2 sorting | |||||||||
|
Cell type / |
Mean |
no. of |
Mean |
population doublings (pds) /day |
|||||
|
medium |
culture time |
passages |
pds |
Unsorted |
W8B2+ |
W8B2- |
|||
| |
[d] |
|
|
mean |
sd |
mean |
sd |
mean |
sd |
| chondrocytes |
70.00 |
4 - 6 |
10.57 |
0.15 |
0.01 |
0.14 |
0.01 |
0.15 |
0.01 |
| n = 3 | |||||||||