Table 1.

NADase activity of S. pyogenes strains

Strain	covS	covR	NADase (Ua)	Reference
1529	wt	wt	3.4 ± 0.7	[23]
1529ΔcovS	ΔcovS	wt	93.5 ± 3.5	this study
K2	I30L	wt	62.9 ± 4.6	this study
GT01	A206S	wt	57.0 ± 3.6	[23]
CR01	M391R	wt	114.3 ± 8.7	[23]
AP04	E428G	wt	59.8 ± 2.6	this study
AP06	E428G	wt	60.5 ± 5.4	this study
FI01	I381T+H437R	wt	59.4 ± 4.8	[23]
1529ΔcovR	wt	ΔcovR	106.7 ± 3.7	this study
GT01ΔcovS	ΔcovS	wt	105.0 ± 3.2	this study
GT01ΔcovR	wt	ΔcovR	103.5 ± 6.7	this study
1529ΔcovS (pLZ12-km2)	ΔcovS	wt	201.9 ± 2.8	this study
1529ΔcovS (pLZ-covS1529)	wt	wt	130.4 ± 3.4	this study
1529ΔcovS (pLZ-covS1529I381L)	I381T	wt	176.7 ± 8.9	this study
1529ΔcovS (pLZ-covS1529 H437R)	H437R	wt	114.5 ± 6.8	this study
1529ΔcovS (pLZ-covS1529I30L)	I30L	wt	162.7 ± 11.0	this study
1529ΔcovS (pLZ-covS1529E428G)	E428G	wt	184.8 ± 6.9	this study
1529ΔcovS (pLZ-covS1529A206S)	A206S	wt	186.7 ± 4.2	this study
1529 (pLZ12-km2)	wt	wt	2.4 ± 0.16	this study

^a NADase activity (Units) ± standard error are indicated. One unit of NADase activity is defined as the amount (μg) of β-NAD cleaved per hour per μl culture supernatant, as described previously [23,31].