Table 1.
Genes and MGE in the genetic context of mecA in WCH1
| Gene or MGE | Position a | Product |
Closest match b,c |
|---|---|---|---|
| Identity, species strain | |||
| orfX |
1-316 |
Hypothetical protein |
99%, S. haemolyticus JCSC1435 |
|
ADP |
445-1431 |
ADP-ribosylglycohydrolase |
99%, S. epidermidis RP62a (locus SERP2218) |
|
perM |
1450-2784 |
Cytosine/purines, uracil, thiamine, allantoin permease family protein |
99%, S. epidermidis RP62a (locus SERP2217) |
|
rbkΔd |
2781-3719 |
Ribokinase |
99%, S. epidermidis RP62a (locus SERP2216) |
| IS431 |
3701-4401 |
IS431 |
|
|
merR |
4888-5235 |
Transcriptional regulator of the merR family |
100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0094) |
|
thiJ |
5313-5996 |
ThiJ/PfpI family protein |
100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0095) |
| orf8 |
6018-6683 |
NAD dependent epimerase/dehydratase family protein |
100%, type IX SCCmec of S. aureus JCSC6943, type X SCCmec of S. aureus JCSC6945 and S. haemolyticus JCSC1435 (locus SH0095) |
| orf9 |
6687-7691 |
Oxidoreductase, zinc-binding dehydrogenase family protein |
100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0097) |
| orf10 |
7700-8065 |
Hypothetical protein of the COG4270 superfamily, predicted membrane protein |
100%, type IX SCCmec of S. aureus JCSC6943, type X SCCmec of S. aureus JCSC6945 and S. haemolyticus JCSC1435 (locus SH0098) |
|
cadD |
8601-9218 |
Cadmium binding protein |
100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0099) |
|
cadX |
9237-9578 |
Cadmium resistant accessory protein |
100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0100) |
|
arsC |
9999-9598 |
Arsenate reductase |
100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0101) |
|
arsB |
11306-10017 |
Arsenical pump membrane protein |
99%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0102) |
|
arsR |
11623-11302 |
Arsenical resistance operon repressor |
100%, type IX SCCmec of S. aureus JCSC6943, type X SCCmec of S. aureus JCSC6945 and S. haemolyticus JCSC1435 (locus SH0103) |
| IS431 |
11697-12486 |
IS431 |
|
|
mecRΔ |
12503-12487 |
Signal transducer protein |
|
|
mecA |
12603-14609 |
Penicillin binding protein 2a |
|
| orf19 |
15083-14655 |
Hypothetical protein |
|
|
maoC |
15923-15180 |
Putative acyl dehydratase maoc |
|
| orf21 |
17208-16840 |
Putative HMG-CoA synthase (partial) |
|
| IS431 |
17209-17998 |
IS431 |
|
|
copA |
18241-20262 |
Copper-transporting atpase |
99%, type X SCCmec of S. aureus JCSC6945. |
| orf24 |
20277-21710 |
Putative multicopper oxidases |
99%, S. haemolyticus JCSC1435 (locus SH0106) |
|
lip |
21730-22212 |
Lipoprotein |
99%, S. aureus JCSC6943 |
|
acf |
22588-23073 |
Putative Acyl-CoA acyltransferase |
97%, S. haemolyticus JCSC1435 (locus SH0117) |
|
hsdR |
23254-23667 |
Type I restriction endonuclease, HsdR |
97%, S. haemolyticus JCSC1435(locus SH0118) |
|
putP |
25274-23736 |
Sodium/proline symporter (High affinity proline permease) |
78%, S. saprophyticus ATCC 15305 (locus SSP0399) |
| IS431Δ |
26462-27184 |
IS431, truncated |
|
|
FAD |
27261-28382 |
FAD-dependent pyridine nucleotide-disulphide oxidoreductase |
66%, a few S. aureus strains, e.g. COL |
|
feoB |
28376-29272 |
FeoB family ferrous iron transporter |
68% (partially, from position 28804 to 29216), S. carnosus TM300 |
| orf31 |
29337-29717 |
Putative transmembrane protein |
73% (partially, from position 29438 to 29618), S. aureus MSHR1132 |
| IS431Δe |
30690-29891 |
IS431, incomplete due to internal termination |
|
| orf32 |
31660-33822 |
ABC-type bacteriocin transporter family protein |
71%, S. epidermidis plasmid SAP105A |
| orf33 |
34541-35809 |
DUF867 type protein, putative phage-related protein |
71% (partially from position 35252), S. epidermidis ATCC 12228 |
| ISSha1 |
37543-36061 |
ISSha1 |
98%, S. haemolyticus JCSC1435 |
|
chr |
38832-37669 |
Chromate transporter |
66% (partially from position 37895 to 38782), Oceanobacillus iheyensis HTE831 |
|
arsC |
39261-38869 |
Arsenate reductase |
97%, S. aureus strains LGA251 and M10/0061 |
|
arsB |
40577-39279 |
Arsenical pump membrane protein |
92%, S. xylosus plasmid pSX267 |
|
arsR |
40885-40571 |
Arsenical resistance operon repressor |
91%, S. aureus plasmid SAP099B and EDINA |
| orf39 |
41223-41771 |
DUF576 type protein |
100%, S. haemolyticus JCSC1435 (locus SH0120) |
| orf40 |
41768-41935 |
Hypothetical protein |
100%, S. haemolyticus JCSC1435 (locus SH0121) |
| orf41 |
42126-43013 |
Hypothetical protein, similar to mechanosensitive ion channel Mscs, transmembrane protein |
100%, S. haemolyticus JCSC1435 (locus SH0122) |
| orf42 |
43522-44046 |
DUF3267 type protein |
100%, S. haemolyticus JCSC1435 (locus SH0123) |
| orf43 |
44998-44120 |
Hypothetical protein, similar to cobalamin synthesis related protein CobW |
100%, S. haemolyticus JCSC1435 (locus SH0124) |
| orf44 | 45625-46248 | Hypothetical protein, similar to Zn-binding lipoprotein AdcA | 100%, S. haemolyticus JCSC1435 (locus SH0125) |
a Positions are according to GenBank accession no. JQ764731.
b GenBank accession no.: S. aureus LGA251 (FR821779), S. aureus JCSC6943 (AB505628), S. aureus JCSC6945 (AB505630), S. aureus M10/0061 (FR823292), S. aureus MSHR1132 (FR821777), S. carnosus TM300 (AM295250), S. epidermidis ATCC 12228 (AE015929), S. epidermidis RP62a (CP000029), S. haemolyticus JCSC1435 (AP006716), S. saprophyticus ATCC 15305 (AP008934), Oceanobacillus iheyensis HTE831 (BA000028), S. aureus plasmid SAP099B (GQ900449), S. aureus plasmid EDINA (AP003089), S. epidermidis plasmid SAP105A (GQ900452), S. xylosus plasmid pSX267 (M80565).
c Closest matches of MGE (IS431 and ISSha1) and genes belonging to the mec complex are not listed as there are many identical matches.
d Truncated by IS431 with 19 bp of the 3′ end missing and the read frame extending into IS431.
e The tnpA of IS431 was terminated prematurely due to internal point mutation.