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. 2013 Mar 22;6:18. doi: 10.1186/1757-2215-6-18

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Copyright © 2013 Park et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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ALDH1(+) cells show stem cell properties. A. mRNA expression of stemness-associated genes, particularly Notch 3, increased in ALDH1(+) cells compared with that in ALDH1(−) cells. The graph represents the relative fold expression of band density normalized to that of GAPDH (*p < 0.05). B. The spheroid formation assay revealed an increased number of spheroids in ALDH1(+) cells. The graph presents the average spheroid formation in ALDH1(+) and ALDH1(−) cells (*p < 0.05). All data are representative of at least two independent experiments.