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Virus neutralisation assay. A mixture of all of the four FP recombinants (4FPmix) was incubated with IHD-J hyperimmune serum to determine whether the proteins expressed by the recombinants were localised on the surface of the new viral progeny. High neutralising activity was detected only in the samples where IHD-J was pre-incubated with IHD-J-hyperimmune mouse serum.
