Table 2.
Official gene symbol | Primer sequences (5′ to 3′) | Primerbank ID | Product size | Efficiency |
---|---|---|---|---|
ACTB (Ref. Gene) |
Fwd: CTCTTCCAGCCTTCCTTCCT |
N/A |
116 bp |
100% |
Rev: AGCACTGTGTTGGCGTACAG | ||||
ADM |
Fwd: ATGAAGCTGGTTTCCGTCG |
4501945a1 |
112 bp |
100% |
Rev: GCCCACTTATTCCACTTCTTTCG | ||||
AQP9 |
Fwd: CTGCAACCGTCTTTGGCATTT |
10280624a3 |
118 bp |
106% |
Rev: AGATACGGAGCTGGGTATGTT | ||||
AREG |
Fwd: GTGGTGCTGTCGCTCTTGATA |
4502199a1 |
171 bp |
97% |
Rev: ACTCACAGGGGAAATCTCACT | ||||
GAPDH (Ref. Gene) |
Fwd: CATGAGAAGTATGACAACAGCCT |
7669492a3 |
113 bp |
97% |
Rev: AGTCCTTCCACGATACCAAAGT | ||||
GJA1 (Connexin 43) |
Fwd: GTGCCTGAACTTGCCTTTTC |
N/A |
165 bp |
98% |
Rev: CCCTCCAGCAGTTGAGTAGG | ||||
IGFBP3 |
Fwd: AGAGCACAGATACCCAGAACT |
4504617a2 |
105 bp |
100% |
Rev: TGAGGAACTTCAGGTGATTCAGT | ||||
NDRG1 |
Fwd: TCGAGACTTTACATGGCTCTGT |
207028746b2 |
93 bp |
106% |
Rev: TCATGCCGATGTCATGGTAGG | ||||
SCGB1A1 (Uteroglobin) |
Fwd: TTCAGCGTGTCATCGAAACCC |
4507809a1 |
189 bp |
100% |
Rev: ACAGTGAGCTTTGGGCTATTTTT | ||||
SPAG6 |
Fwd: GTAAGGTGCTGCCGCATGATA |
6912678a1 |
152 bp |
100% |
Rev: CCTCACTATTTCCTCGGGGTA | ||||
TEKT1 |
Fwd: CAGATTCGGATGAACCGCTCT |
16753231a3 |
140 bp |
103% |
Rev: CTCACGGCGTTCTCAGAATATC | ||||
TMEM45a | Fwd: GTTCACTTCCTGTGTCCTTAACC |
8922242b2 | 95 bp | 97% |
Rev: CATTTCCCGGCCATGAGTGT |
Forward and reverse primers for amplification of the indicated genes were found at Harvard Primerbank (primer IDs shown; N/A indicates in-house primer design). Product sizes and primer efficiencies for Pfaffl calculations are also shown.