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. 2013 Mar 28;14(1):38. doi: 10.1186/1465-9921-14-38

Table 2.

Primers used in qRT-PCR amplification

Official gene symbol Primer sequences (5to 3) Primerbank ID Product size Efficiency
ACTB (Ref. Gene)
Fwd: CTCTTCCAGCCTTCCTTCCT
N/A
116 bp
100%
Rev: AGCACTGTGTTGGCGTACAG
ADM
Fwd: ATGAAGCTGGTTTCCGTCG
4501945a1
112 bp
100%
Rev: GCCCACTTATTCCACTTCTTTCG
AQP9
Fwd: CTGCAACCGTCTTTGGCATTT
10280624a3
118 bp
106%
Rev: AGATACGGAGCTGGGTATGTT
AREG
Fwd: GTGGTGCTGTCGCTCTTGATA
4502199a1
171 bp
97%
Rev: ACTCACAGGGGAAATCTCACT
GAPDH (Ref. Gene)
Fwd: CATGAGAAGTATGACAACAGCCT
7669492a3
113 bp
97%
Rev: AGTCCTTCCACGATACCAAAGT
GJA1 (Connexin 43)
Fwd: GTGCCTGAACTTGCCTTTTC
N/A
165 bp
98%
Rev: CCCTCCAGCAGTTGAGTAGG
IGFBP3
Fwd: AGAGCACAGATACCCAGAACT
4504617a2
105 bp
100%
Rev: TGAGGAACTTCAGGTGATTCAGT
NDRG1
Fwd: TCGAGACTTTACATGGCTCTGT
207028746b2
93 bp
106%
Rev: TCATGCCGATGTCATGGTAGG
SCGB1A1 (Uteroglobin)
Fwd: TTCAGCGTGTCATCGAAACCC
4507809a1
189 bp
100%
Rev: ACAGTGAGCTTTGGGCTATTTTT
SPAG6
Fwd: GTAAGGTGCTGCCGCATGATA
6912678a1
152 bp
100%
Rev: CCTCACTATTTCCTCGGGGTA
TEKT1
Fwd: CAGATTCGGATGAACCGCTCT
16753231a3
140 bp
103%
Rev: CTCACGGCGTTCTCAGAATATC
TMEM45a Fwd: GTTCACTTCCTGTGTCCTTAACC
8922242b2 95 bp 97%
Rev: CATTTCCCGGCCATGAGTGT

Forward and reverse primers for amplification of the indicated genes were found at Harvard Primerbank (primer IDs shown; N/A indicates in-house primer design). Product sizes and primer efficiencies for Pfaffl calculations are also shown.