Table 5.
Genes for which differential expression was reanalysed by qRT-PCR in independent nasal cell samples
| Gene symbol | Affymetrix ID | Gene description | FC (Array) | FC (QPCR) | p (t test) |
|---|---|---|---|---|---|
|
Upregulated in CF |
|
|
|
|
|
|
ADM |
202912_at |
Adrenomedullin |
2.9 |
1.7 |
0.08 |
|
AQP9 |
205568_at |
Aquaporin 9 |
4.9 |
1.8 |
0.41 |
|
AREG |
205239_at |
Amphiregulin |
3.6 |
1.3 |
0.21 |
|
GJA1 |
201667_at |
Gap junction protein alpha 1 or Connexin 43 |
3.4 |
2.7 |
0.22 |
|
IGFBP3 |
210095_s_at |
Insulin-like growth factor binding protein 3 |
2.2 |
1.2 |
0.49 |
|
NDRG1 |
200632_s_at |
N-myc downstream regulated gene 1 |
2.6 |
2.0 |
0.18 |
|
TMEM45a |
219410_at |
Transmembrane protein 45A or DERP7 |
3.0 |
1.0 |
0.5 |
|
Downregulated in CF |
|
|
|
|
|
|
SCGB1A1 |
205725_at |
Secretoglobin family 1A member 1 or Uteroglobin |
−3.3 |
−2.3 |
0.11 |
|
SPAG6 |
210033_s_at |
Sperm associated antigen 6 |
−2.5 |
−3.4 |
0.1 |
|
TEKT1 |
239216_at |
Tektin 1 |
−3.3 |
−3.0 |
0.12 |
|
reference genes |
|
|
|
|
|
|
ACTB |
3 probesets |
Actin beta |
mean 1.2 |
1.2 |
/ |
| GAPDH | 3 probesets | Glyceraldehyde 3-phosphate dehydrogenase | mean 1.1 | 0.8 | / |
Table shows gene symbols and description, Affymetrix probeset ID as represented on the HsAirway array, fold change in CF-vs.-non CF samples for both microarray and qRT-PCR, and p value for qRT-PCR data.