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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Mucosal Immunol. 2012 Aug 29;6(2):379–392. doi: 10.1038/mi.2012.81

Table 1. Summary of Antibodies Used for Immunodetection.

Target Antibody/Staining Epitope/Region Ref
MUC1 B27.29 (mouse IgG1) Recognizes glycosylated N-terminal repeat (PDTRPAP) Ref (62)
214D4 (mouse IgG1) Recognizes unknown epitope at the glycosylated repeat region Ref (63)
MUC4 MUCH4 (Rabbit) Recognizes a peptide (FLNSNSGLQGLQFYR) in un-glycosylated region close to Cys-rich domain (this paper)
IG8 (mouse IgG1) Recognizes intracellular immature molecule (this paper)
MUC5AC MAN5AC (rabbit) Recognizes non-glycosylated C-terminal peptide (RNQDQQGPFKMC) Ref (64)
45M1 (mouse IgG1) C-terminal cystein rich part Ref (67)
MUC5B EU-MUC5Ba (mouse IgG1) Recognizes the peptide (RNREQVGKFKMC) located in repeated cysteine-rich domain Ref (65)
MAN5B/LUM5B (rabbit) Recognizes peptide (ElGQVVECSLDFGLVCR) located in the cysteine repeat Ref (8)
MUC16 OCS125 (Mouse IgG1) Recognizes protein domain in SEA repeat Ref (44)
X325 (Mouse IgG1) Purified protein, Abcam (ab10033) Ref (44)
Muc16 (mouse) mMuc16 (Rabbit) Recognizes the peptide (TSGTESTLISTSAPE) (this paper)
MUC20 MUCH20 (Rabbit) recognizes C-terminal peptide (LSVASPEDLTDPR) Ref (35) and (this paper)
Keratan sulphate 5D4 (Mouse IgG1) Recognizes N-acetyl lactosamine disaccharides of KS with a pentasulfated hexasaccharide. Ref (66)
Carbohydrate AB/PAS (Alcian blue/PAS) AB stains acidic (sulphated) mucins and proteoglycans, PAS stains mucins and, glycoproteins, primarily their terminal sialic acid moieties, but also other carbohydrates including glycogen.