Table 3.

Changes in expression of selected genes related to inflammatory response and autoimmunity in jetPEI^™ or polyplex-exposed HeLa S3 cells.

GeneBank	Symbol	Descriptiona	Fold Change in Gene Expressionb
			jetPEI ™		GFP (CMV) polyplexes		GFP (EF1α) polyplexes
			4h	24h	4h	24h	4h	24h
NM_002990	CCL22	Chemokine (C-C motif) ligand 22	3.6	6.8	5.4	272.8	4.3	213.1
NM_002983	CCL3	Chemokine (C-C motif) ligand 3	−1.2	46.5	−1.1	1084.1	1.9	989.4
NM_002985	CCL5	Chemokine (C-C motif) ligand 5	1.1	2.4	4.5	109.6	4.1	116.2
NM_001295	CCR1	Chemokine (C-C motif) receptor 1	1.4	1.1	1.8	24.2	−2.0	20.1
NM_001565	CXCL10	Chemokine (C-X-C motif) ligand 10	−2.4	2.6	2.3	553.0	2.1	652.0
NM_000575	IL1A	Interleukin 1, alpha	−2.1	3.3	−1.0	9.0	1.1	7.3
NM_000576	IL1B	Interleukin 1, beta	−1.7	2.6	−1.2	7.3	−1.3	6.3
NM_000877	IL1R1	Interleukin 1 receptor, type I	−6.8	1.0	−3.4	3.6	−5.8	3.1
NM_000577	IL1RN	Interleukin 1 receptor antagonist	1.4	2.0	−1.1	4.6	1.0	6.0
NM_016584	IL23A	Interleukin 23, alpha subunit p19	−1.2	1.1	1.2	7.2	−1.6	6.1
NM_000600	IL6	Interleukin 6 (interferon, beta 2)	1.4	1.6	7.5	11.8	7.1	12.4
NM_003263	TLR1	Toll-like receptor 1	−5.8	1.2	−1.6	3.4	−2.4	2.8
NM_003265	TLR3	Toll-like receptor 3	−2.2	1.1	−1.9	11.5	−1.7	10.8
NM_138554	TLR4	Toll-like receptor 4	1.1	1.6	−1.2	3.9	−1.3	4.3
NM_003268	TLR5	Toll-like receptor 5	1.2	−6.5	1.5	5.8	1.5	9.6
NM_000594	TNF	Tumor necrosis factor (TNF superfamily, member 2)	13.0	1.2	18.6	14.9	14.6	14.1

^aThe gene descriptions are reproduced from the array product information from Qiagen.

^bGreen and red shading indicate fold-changes of ≥ 2 and ≤ 2, respectively, with p ≤ .05.