Fig. 2.

3′UTR length heterogeneity as observed using 3′RACE-PCR. RNA was isolated from wildtype mouse organs, mouse primary cortical neurons, and human MCF-7 cells were used to perform 3′ Rapid Amplification of cDNA 3′ Ends (RACE) PCR using APP-specific oligonucleotides. These assays reveal changes in APP 3′UTR length and abundance depending on developmental stage, age, cell type and organ. Arrows indicate various 3′UTR products. Different 3′UTRs for a given transcript is probably one of many ways that miRNA targeting is regulated in the brain.