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. 2013 Apr 8;110(17):E1631–E1640. doi: 10.1073/pnas.1219542110

Fig. 4.

Fig. 4.

Effects of selective RIIβ expression in D1R or D2R neurons on locomotion and adiposity. (A) Immunostaining of RIIβ in brain sections of HET, RIIβlox/−, D1R-Cre/RIIβlox/− (RIIβD1R), and D2R-Cre/RIIβlox/− (RIIβD2R) mice. (B) Immunoblots and quantification of RIIβ level in the striatum of HET, RIIβlox/−, RIIβD1R, and RIIβD2R mice. ***P < 0.001, unpaired t test compared with HET controls (n = 3 for each genotype). (C and D) Locomotor activity (black bar in C indicates dark cycle), (E) body weight, (F) reproductive fat-pad weight, and (G) serum leptin level of HET, RIIβlox/−, RIIβD1R, and RIIβD2R mice at 16–20 wk of age (n = 8–12 for each group). Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired t test compared with HET control or as indicated.