Fig. 3.

DEK taken up by cells migrates to chromatin. (A) Twelve-day MDMs in 40% human serum were treated overnight with 15 µg of recombinant His-tagged DEK. Cells were stained for DEK with a monoclonal anti-His antibody, and nuclei were stained with propidium iodide (PI). (B) Immunoprecipitation of DEK from cytoplasmic and nuclear fractions of cells treated as in A with a rabbit polyclonal anti-His antibody. The resulting samples were analyzed on an SDS gel and immunoblotted with anti-DEK or anti-His monoclonal antibodies. (C) Control or HeLa DEK-KD cells were incubated with recombinant His-DEK or GST-DEK for 24 h and fractionated into cytosolic, nucleosolic, and chromatin-bound fractions. Proteins from each fraction were precipitated, resolved by SDS/PAGE, and blotted for DEK using a polyclonal antibody.