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Testing uniRapR in different kinases and effects of Src activation in HeLa cells. Immunoprecipitation, in vitro FAK (A), and p38 (B) assays were performed similarly as for Src kinase. (C) Change in cell area of HeLa cells expressing Src (YF)-uniRapR (n = 8/8 cells) or Src (YF/KD)-uniRapR (n = 8/8 cells). (D) HeLa cells expressing Src-uniRapR (YF)-cerulean demonstrate spreading after the addition of rapamycin.
