Three-dimensional reconstruction of MDA and 8-OHdG immunoreactivities Cut view images after TBI challenge. The micrographs highlight that 8-OHdG immunoreactivity deposits in microvessels-like structures, while MDA are anchored to Purkinje cells and granular cells (A). B: A series of digital images (1 tm) acquired by Z-stack scanning from the bottom to the top of to Purkinje cells and granular cells. Green, MDA; Red, 8-OHdG, blue, DAPI. C: A 3D structure of three layers, molecular layer, Purkinje cell layer and granular layer in cerebella.