Figure 7. Rescue of NSCs maintenance in FIP200^hGFAP cKO mice by scavenging abnormally elevated ROS.

(A-C) H&E staining of the SVZ and DG of P28 mice treated by NAC or vehicle control. Lines indicate boundaries for the SVZ and DG. Arrows mark cells in the SVZ. Mean±SE of the SVZ cellularity (B) and DG area (C) per section are shown. (D-M) Immunofluorescence of the DG (D-H) and SVZ (I-M) of P28 mice treated by NAC or vehicle control. Representative images are shown in D, G (DG) and K, M (SVZ). Lines indicate boundaries of the GZ (D, G) and SVZ (K, M). The boxed areas in G, K and M are shown in more detail in insets (G) or panels below (K and M). Lines indicate boundaries of the GZ (D, G) and SVZ (K, M). Arrows mark GFAP⁺/Nestin⁺ and GFAP⁺/SOX2⁺ NSCs with radial glial morphology (D, G), and arrowheads mark GFAP⁺/Nestin⁻ and GFAP+/SOX2⁻ astrocytes. Mean±SE of the number of GFAP⁺/Nestin⁺ and GFAP⁺/SOX2⁺ radial glia (E, H), and NSCs (I, L), and GFAP⁺/Nestin⁻ astrocytes (F, J) per section are shown. (N, O) Mean±SE of the number of TUNEL⁺ cells per 100 SVZ cells (N) or per 1 mm² DG area (O) of P28 mice treated by NAC or vehicle control are shown. n= 5 mice, ≥4 sections/mouse, >500 cells counted/mouse in N. For all panels, cKO(n): NAC non-responder cKO mice.