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. Author manuscript; available in PMC: 2013 Apr 28.
Published in final edited form as: Nature. 1999 Jun 10;399(6736):597–601. doi: 10.1038/21218

Figure 3.

Figure 3

Evidence that Ser 1179 is functionally important for Akt-stimulated NO release. COS cells were transfected with plasmids for wild-type eNOS or eNOS mutants, in the absence or presence of Akt, and the expression of the proteins and production of NO (assayed as NO2−) were determined. Constructs with the S1179A mutation were not activated by Akt; the S1179D mutation resulted in a gain of function. Data are mean ± s.e.m. of 4-7 experiments; asterisks represent significant differences (P < 0.05).