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. Author manuscript; available in PMC: 2013 Apr 28.
Published in final edited form as: Nat Genet. 2011 Dec 4;44(1):23–31. doi: 10.1038/ng.1009

Figure 3.

Figure 3

Dnmt3a-null HSCs show inhibition of long-term differentiation in serial competitive transplantation of HSCs. (a) The proportion of peripheral blood generated from the test cells in recipient mice 16 weeks after transplantation. (b) Quantification of donor-derived HSCs in the bone marrow of recipient mice 18 weeks after transplantation, defined as CD45.2+, SPKLS cells. Data are representative of at least three individual transplantation experiments for each stage of serial transfer (N = 15–37 mice per group). Mean ± s.e.m. values are shown. (c) Flow cytometry data of quaternary recipient mice transplanted with control or Dnmt3a-null HSCs showing virtually all continuously amplified HSCs in bone marrow were derived from Dnmt3a-null HSCs (CD45.2+). (d) Differentiation and self-renewal quotients, calculated at the end of each round of transplantation with Dnmt3a-null and control HSCs. ***P < 0.001.