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. Author manuscript; available in PMC: 2013 Apr 28.
Published in final edited form as: Nat Genet. 2011 Dec 4;44(1):23–31. doi: 10.1038/ng.1009

Figure 7.

Figure 7

Exogenous Dnmt3a partially restores function and methylation patterns. (a) Analysis of HSC (CD150+, CD48−, KLS gated) frequency 18-weeks after transplantation. Sca-1+ cells from secondary recipient mice transplanted with Dnmt3a-null HSCs were transduced with either MSCV-Dnmt3a or control (MSCV-GFP) retroviruses and transplanted into tertiary recipients. Also shown are HSC frequencies in tertiary recipients transplanted with non-transduced control or Dnmt3a-null HSCs. Mean ± s.e.m. values are shown (N = 7–12 mice), and statistically significant differences are indicated. (b) Colony-forming capacity of Dnmt3a-null HSCs transduced with MSCV-GFP or MSCV-Dnmt3a. Also shown are colony formation from non-transduced control and Dnmt3a-null HSCs after the third serial transplantation. Mean ± s.e.m. values are shown (N = 4 replicate plates), and statistically significant differences are indicated. (c) Bisulfite sequencing in B cells across Vasn and Runx1 CGIs after forced exogenous Dnmt3a expression in Dnmt3a-null HSCs. Statistically significant differences in methylation are indicated. *P < 0.05, **P < 0.01, ***P < 0.001.