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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Probiotics Antimicrob Proteins. 2013 Jan 13;5(1):26–35. doi: 10.1007/s12602-012-9123-x

Figure 6. Viral protein gD synthesis is not affected by subtilosin treatment.

Figure 6

Vero cells infected (Lanes 2–5) or not (Lane 1) with HSV-1 tk+ at a m.o.i. of 1 were incubated at 37°C for 1 h to allow internalization. Afterwards, the inoculum was discarded and the monolayers were covered with MM or MM containing subtilosin at different concentrations (Lane 2: virus control; lane 3: 25 µg/mL; lane 4: 50 µg/mL; lane 5: 100 µg/mL) and incubated at 37°C. At 24 h p.i. cell lysates were collected and subjected to Western blotting and glycoprotein gD was analyzed with an antibody against gD. To verify equal loading, Western blotting was performed with an antibody to ERK1. Data are from one of two different experiments.