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The multi-frequency lifetime measurements were quantified after fitting the data to two lifetime components to characterize ECFP's chromophore. The average lifetimes are listed in the table, reflecting an average FRET efficiency near 0.75
| a1 | τ1 (ns) | a2 | τ2 (ns) | ‹τ› (ns) | |
|---|---|---|---|---|---|
| ECFP-RET | 0.92 | 0.42 | 0.08 | 3.99 | 0.71 |
| ECFP | 0.44 | 1.24 | 0.56 | 4.08 | 2.83 |
