Fig. 1.
High performance liquid chromatography-electrospray ionization-tandem mass spectrometry multiple reaction monitoring (negative ion mode) chromatographic profiles of estrogen steroids corresponding to 100 pg/mL in standard solution (4%(w/w) bovine albumin). Chromatograms were obtained using two periods. Period I: 0→5.50 min; Period II: 5.50→8.00 min.