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. Author manuscript; available in PMC: 2013 Apr 28.
Published in final edited form as: Clin Biochem. 2008 Mar 7;41(9):736–741. doi: 10.1016/j.clinbiochem.2008.02.009

Table 1.

MRM conditions for the estrogen steroids and deuterium-labeled internal standard in negative ion mode

Period (min) Estrogen steroid MRM transition Retention time (min) DP EP CE CXP
Period I (5.5) Estriol 287/171 4.46 −120 −10 −55 −15
Estriol (2nd) 287/145 4.46 −120 −10 −55 −15
Estriol-d2 289/147 4.45 −120 −10 −55 −15
16-Hydroxyestrone 285/145 4.77 −120 −10 −55 −15
16-Hydroxyestrone (2nd) 285/143 4.77 −120 −10 −78 −17
Aldosterone-d6 365/337 4.63 −60 −10 −23 −15
Period II (2.5) Estrone 269/145 6.88 −120 −10 −55 −15
Estrone (2nd) 269/143 6.88 −120 −10 −55 −15
Estrone-d4 273/147 6.86 −120 −10 −55 −15
Estradiol 271/145 6.93 −120 −10 −55 −15
Estradiol (2nd) 271/183 6.93 −120 −10 −55 −15
Estradiol-d4 275/147 6.89 −120 −10 −55 −15

Note: for estriol, 16-hydroxyestrone, estrone, and estradiol, the first MRM transitions were listed for quantitation, and the second transitions were added for confirmation.