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. 2013 Apr 15;14:255. doi: 10.1186/1471-2164-14-255

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Copyright © 2013 Veneman et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Invasion of S. epidermidis into the zebrafish embryo body. Confocal z-stacks are shown as transmission/fluorescence overlay (a &c) and fluorescence images (b &d). Panel 1: at 3 DPI mCherry labelled S. epidermidis is observed inside the body (1a &1b, scale bar: 50 μm), and intracellular in the hematopoietic region (1c &1d, scale bar: 10 μm). Panel 2: at 4 DPI bacteria are found inside the vasculature (2a &2b, scale bar: 50 μm), including the intersegmental vessels (2d &2d, scale bar: 10 μm). Panel 3: at 5 DPI bacteria are still persisting in the vasculature (3a &3b, scale bar: 25 μm) and in the intersegmental vessels (3c &3d, scale bar: 10 μm). Panel 4: bacteria being taken up by mpeg1:KAEDE positive cells and extracellular in the hematopoietic region at 3 DPI (scale bar: 10 μm).