Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Apr 15;14:255. doi: 10.1186/1471-2164-14-255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Veneman et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Workflow of high throughput injection and subsequent analysis. Panel A: from left to right; a zebrafish pair are put together to mate, eggs are collected, eggs are distribute into a 1024 well agarose grid, eggs are injected into the yolk at 2 HPF using the automated microinjection system. Panel B: from left to right; after injection, eggs are collected into Petri dishes and incubated at 28°C for a period of 5 days, COPAS analysis is performed on the S. epidermidis and non-injected embryos at 2, 3, 4 and 5 DPI. Panel C: from left to right; from all groups 20 embryos are snap frozen at 6 HPI, 1, 2, 3, 4 and 5 DPI for RNA isolation, amplification and Cy3 labelling, micro-array analysis against Cy5 labelled common reference and data analysis using Rosetta Resolver. Panel D: from left to right; validation of micro-array data was performed by RNAseq analysis of 4 biological replicas of S. epidermidis infected embryos at 5 DPI.