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. Author manuscript; available in PMC: 2014 Feb 5.
Published in final edited form as: Anal Chem. 2013 Jan 14;85(3):1896–1904. doi: 10.1021/ac303188j

Table 2.

Limits of detection (LOD), quantification (LOQ), calibration results and retention times for ARV drugs and metabolites in meconium by LC-MS/MS

Analyte Internal
Standard
LOD
(ng/g)
LOQ
(ng/g)
Slope
(mean ± SD,
n=5)
y-intercept
(mean ± SD,
n=5)
R2
(range, n=5)
Linear
range
(ng/g)
Retention
Time
(min)
TFV TFV-d6 5 10 1.812 ± 0.087 0.002 ± 0.031 0.997–0.999 10–2500 4.3
3TC 3TC-13C,15N2 25 50 1.200 ± 0.112 0.003 ± 0.004 0.995–0.998 50–2500 5.5
FTC FTC-13C,15N2 5 10 1.306 ± 0.046 −0.003 ± 0.004 0.998–1.000 10–2500 6.5
ABC ABC-d4 1 10 0.480 ± 0.028 0.005 ± 0.004 0.993–0.999 10–2500 11.5
ABC-G ABC-d4 5 10 0.148 ± 0.043 0.000 ± 0.001 0.990–0.995 10–2500 10.5
ABC-C ABC-d4 25 75 0.015 ± 0.003 0.002 ± 0.001 0.990–0.998 75–2500 11.6
NVP NVP-d5 5 10 1.65 ± 0.336 0.523 ± 0.032 0.990–0.999 10–2500 13.9
RAL RAL-d3 10 10 1.628 ± 0.106 −0.031 ± 0.015 0.998–1.000 10–2500 17.5
APV APV-d4 10 100 0.514 ± 0.033 0.009 ± 0.004 0.994–1.000 100–25000 18.6
DRV DRV-d9 50 100 0.940 ± 0.337 0.002 ± 0.002 0.996–0.998 100–25000 18.7
M8 NFV-d3 5 10 7.368 ± 0.060 0.107 ± 0.060 0.994–0.998 10–2500 18.9
NFV NFV-d3 5 10 5.020 ± 0.360 0.105 ± 0.036 0.996–0.999 10–2500 20.1
SQV SQV-d9 5 10 0.727 ± 0.048 0.006 ± 0.009 0.994–0.999 10–2500 20.9
ATV ATV-d5 1 10 1.898 ± 0.136 0.175 ± 0.009 0.995–0.999 10–2500 22.4
RTV RTV-d6 1 10 1.684 ± 0.033 0.052 ± 0.033 0.996–0.999 10–2500 23.3
LPV LPV-d8 5 10 0.795 ± 0.014 0.139 ± 0.005 0.996–0.999 10–2500 24.1
d4T d4T-d3 250 500 0.531 ± 0.172 0.007 ± 0.007 0.994–0.998 500–25000 4.3
AZT AZT-d3 50 100 0.860 ± 0.170 0.005 ± 0.009 0.996–0.999 100–25000 4.9
AZT-G AZT-G-d3 500 500 2.072 ± 0.132 −0.117 ± 0.036 0.990–0.993 500–25000 5.3
EFV EFV-d5 50 100 1.698 ± 0.515 0.003 ± 0.004 0.997–0.999 100–25000 8.0