Table 2.
Analyte | Internal Standard |
LOD (ng/g) |
LOQ (ng/g) |
Slope (mean ± SD, n=5) |
y-intercept (mean ± SD, n=5) |
R2 (range, n=5) |
Linear range (ng/g) |
Retention Time (min) |
---|---|---|---|---|---|---|---|---|
TFV | TFV-d6 | 5 | 10 | 1.812 ± 0.087 | 0.002 ± 0.031 | 0.997–0.999 | 10–2500 | 4.3 |
3TC | 3TC-13C,15N2 | 25 | 50 | 1.200 ± 0.112 | 0.003 ± 0.004 | 0.995–0.998 | 50–2500 | 5.5 |
FTC | FTC-13C,15N2 | 5 | 10 | 1.306 ± 0.046 | −0.003 ± 0.004 | 0.998–1.000 | 10–2500 | 6.5 |
ABC | ABC-d4 | 1 | 10 | 0.480 ± 0.028 | 0.005 ± 0.004 | 0.993–0.999 | 10–2500 | 11.5 |
ABC-G | ABC-d4 | 5 | 10 | 0.148 ± 0.043 | 0.000 ± 0.001 | 0.990–0.995 | 10–2500 | 10.5 |
ABC-C | ABC-d4 | 25 | 75 | 0.015 ± 0.003 | 0.002 ± 0.001 | 0.990–0.998 | 75–2500 | 11.6 |
NVP | NVP-d5 | 5 | 10 | 1.65 ± 0.336 | 0.523 ± 0.032 | 0.990–0.999 | 10–2500 | 13.9 |
RAL | RAL-d3 | 10 | 10 | 1.628 ± 0.106 | −0.031 ± 0.015 | 0.998–1.000 | 10–2500 | 17.5 |
APV | APV-d4 | 10 | 100 | 0.514 ± 0.033 | 0.009 ± 0.004 | 0.994–1.000 | 100–25000 | 18.6 |
DRV | DRV-d9 | 50 | 100 | 0.940 ± 0.337 | 0.002 ± 0.002 | 0.996–0.998 | 100–25000 | 18.7 |
M8 | NFV-d3 | 5 | 10 | 7.368 ± 0.060 | 0.107 ± 0.060 | 0.994–0.998 | 10–2500 | 18.9 |
NFV | NFV-d3 | 5 | 10 | 5.020 ± 0.360 | 0.105 ± 0.036 | 0.996–0.999 | 10–2500 | 20.1 |
SQV | SQV-d9 | 5 | 10 | 0.727 ± 0.048 | 0.006 ± 0.009 | 0.994–0.999 | 10–2500 | 20.9 |
ATV | ATV-d5 | 1 | 10 | 1.898 ± 0.136 | 0.175 ± 0.009 | 0.995–0.999 | 10–2500 | 22.4 |
RTV | RTV-d6 | 1 | 10 | 1.684 ± 0.033 | 0.052 ± 0.033 | 0.996–0.999 | 10–2500 | 23.3 |
LPV | LPV-d8 | 5 | 10 | 0.795 ± 0.014 | 0.139 ± 0.005 | 0.996–0.999 | 10–2500 | 24.1 |
d4T | d4T-d3 | 250 | 500 | 0.531 ± 0.172 | 0.007 ± 0.007 | 0.994–0.998 | 500–25000 | 4.3 |
AZT | AZT-d3 | 50 | 100 | 0.860 ± 0.170 | 0.005 ± 0.009 | 0.996–0.999 | 100–25000 | 4.9 |
AZT-G | AZT-G-d3 | 500 | 500 | 2.072 ± 0.132 | −0.117 ± 0.036 | 0.990–0.993 | 500–25000 | 5.3 |
EFV | EFV-d5 | 50 | 100 | 1.698 ± 0.515 | 0.003 ± 0.004 | 0.997–0.999 | 100–25000 | 8.0 |