Table 3.
Analytical recovery (bias) and imprecision data for ARVs in meconium
| Analytical Recovery (% of target concentration) | Imprecision (% RSD, n=20) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Analyte | Expected conc. (ng/g) |
Intra-day, n=4 | Inter-day, n=20 | ||||||
| Mean | Range | Mean | Range | Pooled Intra-day |
Inter- day |
Total | |||
| 30 | 107.5 | 100.7 – 117.0 | 105.0 | 86.3 – 117.0 | 4.7 | 7.7 | 9.0 | ||
| TFV | 300 | 106.2 | 101.0 – 114.0 | 107.9 | 101.0 – 117.0 | 3.5 | 3.8 | 5.2 | |
| 2000 | 101.4 | 98.0 – 105.5 | 103.8 | 98.0 – 115.0 | 4.1 | 1.0 | 4.2 | ||
| 150 | 110.3 | 106.7 – 114.7 | 106.4 | 98.0 – 114.7 | 3.5 | 2.0 | 4.0 | ||
| 3TC | 750 | 107.9 | 102.0 – 111.3 | 106.7 | 100.4 – 112.0 | 3.7 | 0.8 | 3.8 | |
| 2000 | 106.5 | 105.0 – 107.5 | 103.7 | 96.0 – 114.5 | 3.5 | 3.1 | 4.7 | ||
| 30 | 105.7 | 104.7 – 106.7 | 103.0 | 96.3 – 111.0 | 2.7 | 1.4 | 3.0 | ||
| FTC | 300 | 103.8 | 101.0 – 108.0 | 104.5 | 96.3 – 111.3 | 3.0 | 1.4 | 3.3 | |
| 2000 | 98.6 | 96 – 101.5 | 99.6 | 90.5 – 108.0 | 3.8 | 3.1 | 5.0 | ||
| 30 | 107.8 | 102.0 – 114.7 | 111.9 | 98.7 – 119.0 | 4.6 | 1.1 | 4.7 | ||
| ABC | 300 | 110.9 | 108.3 – 116.7 | 109.2 | 97.0 – 118.3 | 3.6 | 5.3 | 6.4 | |
| 2000 | 96.9 | 84.0 – 102.5 | 95.3 | 84.0 – 104.5 | 5.4 | 3.3 | 6.3 | ||
| 30 | 120.3 | 105.7 – 127.7 | 109.7 | 88.0 – 127.7 | 8.1 | 8.6 | 11.9 | ||
| ABC-Ga | 300 | 118.8 | 104.0 – 124.3 | 110.8 | 81.3 – 129.3 | 12.4 | 4.1 | 13.1 | |
| 2000 | 114.1 | 96.5 – 122.0 | 102.8 | 72.5 – 137.5 | 15.5 | 9.0 | 17.9 | ||
| 150 | 120.2 | 108.7 – 128.0 | 105.1 | 70.0 – 128.0 | 10.2 | 13.0 | 16.5 | ||
| ABC-Ca | 750 | 120.6 | 112.0 – 133.3 | 111.8 | 91.5 – 137.3 | 11.9 | 5.8 | 13.2 | |
| 2000 | 111.8 | 104.5 – 117.0 | 103.4 | 73.5 – 138.5 | 12.8 | 8.7 | 15.5 | ||
| 30 | 108.3 | 104.3 – 115.3 | 111.0 | 104.3 – 116.7 | 3.6 | 0.7 | 3.7 | ||
| NVP | 300 | 109.4 | 100.7 – 117.7 | 109.7 | 98.3 – 117.7 | 4.5 | 1.1 | 4.7 | |
| 2000 | 90.8 | 86.5 – 95.0 | 92.0 | 84.0 – 99.5 | 2.9 | 3.1 | 4.2 | ||
| 30 | 84.5 | 80.7 – 90.0 | 89.3 | 80.3 – 108.0 | 4.2 | 6.2 | 7.5 | ||
| RAL | 300 | 103.0 | 93.0 – 112.3 | 104.4 | 93.0 – 115.0 | 3.9 | 5.4 | 6.7 | |
| 2000 | 95.0 | 94.0 – 96.0 | 97.3 | 93.5 – 108.0 | 4.0 | 2.6 | 4.8 | ||
| 30 | 105.4 | 84.0 – 119.3 | 104.8 | 84.0 – 119.3 | 6.8 | 8.6 | 11.0 | ||
| M8 | 300 | 103.9 | 86.0 – 119.7 | 103.3 | 86.0 – 119.7 | 6.3 | 7.4 | 9.7 | |
| 2000 | 91.8 | 80.5 – 112.0 | 95.8 | 80.5 – 112.0 | 9.2 | 3.7 | 9.9 | ||
| 300 | 102.8 | 94.3 – 111.7 | 106.1 | 94.0 – 116.0 | 5.3 | 3.4 | 6.3 | ||
| APV | 3000 | 105.6 | 100.3 – 110.0 | 104.7 | 94.3 – 110.0 | 2.9 | 2.2 | 3.6 | |
| 20000 | 89.9 | 86.5 – 93.0 | 92.3 | 84.0 – 104.0 | 3.9 | 0 | 3.9 | ||
| 300 | 96.5 | 88.7 – 108.7 | 101.0 | 88.7 – 108.7 | 4.4 | 2.3 | 4.9 | ||
| DRV | 3000 | 100.1 | 92.7 – 105.3 | 99.7 | 92.7 – 105.3 | 2.4 | 0 | 2.4 | |
| 20000 | 85.5 | 81.0 – 94.5 | 89.2 | 81.0 – 100.0 | 2.8 | 4.3 | 5.1 | ||
| 30 | 105.8 | 102.7 – 109.7 | 107.1 | 93.7 – 116.0 | 4.5 | 2.2 | 5.0 | ||
| NFV | 300 | 101.2 | 94.0 – 107.7 | 106.3 | 94.0 – 118.7 | 3.9 | 4.0 | 5.6 | |
| 2000 | 91.0 | 83.5 – 100.0 | 94.9 | 83.5 – 107.5 | 5.3 | 5.4 | 7.5 | ||
| 30 | 95.5 | 86.7 – 109.7 | 103.0 | 86.7 – 119.0 | 8.1 | 3.6 | 8.9 | ||
| SQV | 300 | 99.4 | 93.7 – 101.7 | 105.5 | 92.0 – 117.0 | 6.0 | 3.4 | 6.9 | |
| 2000 | 101.8 | 90.0 – 103.5 | 99.0 | 90.0 – 114.5 | 6.9 | 0 | 6.9 | ||
| 30 | 107.0 | 101.3 – 114.7 | 110.3 | 101.3 – 119.3 | 3.2 | 3.7 | 4.9 | ||
| ATV | 300 | 103.9 | 100 – 110.7 | 107.0 | 100.0 – 116.7 | 3.1 | 3.7 | 4.8 | |
| 2000 | 89.5 | 84.5 – 93.5 | 91.9 | 84.5 – 101.0 | 2.7 | 2.0 | 3.4 | ||
| 30 | 102.1 | 97.3 – 105.0 | 104.4 | 94.7 – 115.3 | 3.5 | 4.8 | 5.9 | ||
| RTV | 300 | 105.2 | 98.0 – 118.7 | 107.9 | 98.0 – 118.7 | 3.9 | 0 | 3.9 | |
| 2000 | 93.9 | 87.0 – 102.0 | 97.5 | 87.0 – 103.5 | 4.2 | 2.5 | 4.9 | ||
| 30 | 107.3 | 100.7 – 112.3 | 107.7 | 99.3 – 117.7 | 3.7 | 1.3 | 3.9 | ||
| LPV | 300 | 108.8 | 106.3 – 113.3 | 106.5 | 101.3 – 113.3 | 2.3 | 1.3 | 2.7 | |
| 2000 | 98.8 | 90.5 – 103.0 | 99.0 | 90.5 – 103.0 | 3.0 | 0.3 | 3.0 | ||
| 500 | 97.3 | 96.0 – 99.3 | 97.7 | 91.3 – 106.0 | 3.3 | 1.6 | 3.7 | ||
| d4T | 7500 | 100.0 | 98.7 – 103.2 | 100.0 | 92.9 – 105.3 | 2.6 | 2.0 | 3.3 | |
| 20000 | 93.5 | 87.0 – 96.5 | 95.8 | 87.0 – 101.5 | 3.0 | 1.8 | 3.5 | ||
| 300 | 102.2 | 96.0 – 108.3 | 102.0 | 91.3 – 110.0 | 4.1 | 0.9 | 4.2 | ||
| AZT | 3000 | 104.7 | 101.0 – 108.0 | 103.0 | 95.0 – 112.3 | 3.6 | 2.2 | 4.2 | |
| 20000 | 95.5 | 93.0 – 97.5 | 97.8 | 87.5 – 109.0 | 3.2 | 4.4 | 5.4 | ||
| 500 | 95.8 | 92.0 – 100.0 | 98.2 | 92.0 – 101.3 | 1.6 | 1.4 | 2.2 | ||
| AZT-G | 7500 | 102.9 | 96.9 – 107.9 | 107.8 | 96.9 – 117.5 | 4.0 | 1.6 | 4.3 | |
| 20000 | 108.9 | 97.5 – 116.5 | 112.9 | 93.0 – 118.0 | 6.9 | 0 | 6.9 | ||
| 300 | 98.4 | 97.0 – 102.3 | 103.4 | 97.0 – 111.0 | 3.0 | 2.7 | 4.0 | ||
| EFV | 3000 | 103.5 | 101.7 – 106.0 | 104.6 | 99.0 – 111.7 | 2.1 | 2.1 | 3.0 | |
| 20000 | 95.8 | 90.0 – 101.5 | 93.7 | 85.5 – 101.5 | 3.6 | 1.1 | 3.8 | ||
a
Since accuracy fell outside 80–120% target concentrations for ABC-G and ABC-C QC concentrations, these analytes are considered semiquantitative.