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. Author manuscript; available in PMC: 2013 Apr 28.
Published in final edited form as: Clin Cancer Res. 2011 Oct 28;17(24):7595–7604. doi: 10.1158/1078-0432.CCR-11-1456

Figure 1.

Figure 1

BRAF, CRAF, and downstream signaling mediator activation in MA cell lines. A. Cell lysates from 20 human MA cell lines were examined by Western Blot using antibodies against the indicated proteins. Cell lines harboring mutant BRAF are indicated by the dotted line. BRAF protein signals were normalized against corresponding α-TUBULIN signals, with ratios expressed in relation to a normal human astrocyte (Clonetics) value of 100. A second NHA cell source (AllCells) was determined as expressing nearly identical BRAF as the Clonetics NHAs that were used for establishing MA cell line BRAF expression levels.