Figure 2.

Lentivirus-mediated shRNA stably decreases SMC1A expression in U251 cells. A. GFP expression in 4 day old cultures of control U251 cells or cells infected with SMC1A-Scr lentivirus or lentivirus harboring SMC1A-shRNAs (SMC1A#1, #2 or #3). Top, bright field; bottom, GFP signal. B. Representative western blot showing SMC1A levels in control U251 cells and U251 cells expressing SMC1A-shRNA. GAPDH included as a loading control. C. Quantification of real-time RT-PCR measurements of SMC1A expression normalized to β-actin in U251 cells infected as indicated. The protein and RNA were extracted at 4 days after subculture.