Fig. 4.

Distribution of Cx43 and Cx32 in human spinal cord sections obtained from normal individuals and individuals with MS. Confocal microscopy of Cx43 (FITC, green) and MBP (Cy3, red) staining, colocalization of both proteins is represented as orange staining in the last panel. A, B and C, represents staining of human tissue sections for GFAP (FITC, green) and Cx43 (Cy3, red), a small insert shows the Cx43 staining alone, from normal (a), MS with chronic active lesions (b) and chronic silent lesions (c). d, e and f, represents staining for Cx32 (FITC, Green) and MBP (Cy3, red) in human sections obtained from spinal cords from individuals with normal tissue (d), MS with chronic active lesions (e) and silent lesions (f). The small inserts in each picture show the Cx32 staining alone. These tissue sections were already characterized for the kind of MS lesions and the damage in the lesion area (Calderon et al. 2006). Note that in MS tissue it is possible observe oligodendrocyte atrophy and disorganization of the brain parenchyma. Bar: 70 μm