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. Author manuscript; available in PMC: 2014 Mar 28.
Published in final edited form as: Cell. 2013 Mar 28;153(1):139–152. doi: 10.1016/j.cell.2013.02.021

Figure 4. Pericytes Are Commonly Derived from Neoplastic Cells in Primary GBMs.

Figure 4

(A and B) FISH analyses of genetic alterations with the CEP-7, CEP-10, EGFR or PTEN probe (in red) in pericytes (α-SMA+) in primary GBMs. Quantification shows average fractions of pericytes carrying the cancer genetic alterations (CEP-7 polysomy; EGFR amplification or trisomy; CEP-10 loss; or PTEN loss) in GBM tissue arrays (B).

(C and D) FISH analyses with CEP-7 probe in sorted pericytes (α-SMA+) and GSCs from primary GBMs. Quantification shows the fraction (mean 72%) of pericytes carrying the GSC genetic alterations (D).

(E and F) Imf staining of a pericyte marker (Desmin or α-SMA) and the HA-tagged PDGFB in the genetically engineered mouse GBMs (Nestin-tva/Ink4a/Arf−/−/HA-PDGFB model). Quantifications show fractions (mean 63%) of HA-PDGFB+ pericytes (F)

The scale bars represent 10 μm (A and C) and 25 μm (E). The error bars represent SD. See also Figure S5.