Skip to main content
. Author manuscript; available in PMC: 2014 Mar 28.
Published in final edited form as: Cell. 2013 Mar 28;153(1):139–152. doi: 10.1016/j.cell.2013.02.021

Figure 6. GSCs Are Recruited toward ECs through the SDF-1/CXCR4 Axis to Support Endothelial Complex.

Figure 6

(A) In vitro endothelial complex formation of HBMECs (labeled with red fluorescent tracer CMTRX) with or without GSCs (labeled with CFSE, in green).

(B) Imf staining of α-SMA and N-Cadherin in complexes of HBMECs and GSC-derived cells. Nuclei were stained with DAPI.

(C and D) Endothelial complexes of HBMECs with GFP-labeled GSCs expressing shCXCR4) or shNT. Quantification shows fractions of GSC-derived cells (GFP+) on HBMEC complexes (D). *, p < 0.001.

(E-G) In vivo lineage tracing of GSCs with GFP constitutive expression and Imf staining of CD31 or Desmin in tumors derived from GSCs expressing shCXCR4 or shNT. Quantifications show fractions of GSC-derived pericytes (GFP+) (F) and total pericyte coverage (G) on vessels. *, p < 0.001.

The scale bars represent 100 μm (A-C) and 25 μm (E). The error bars represent SD. See also Figure S7.