FIG 5 .
Expression and production of T6SS-associated Hcp and T3SS effector AexU in A. hydrophila strains SSU, ATCC 7966T, E1, and E2. (A) While only SSU secretes Hcp into the supernatant, the Hcp protein is found in the cell pellet of strains E1 and E2. (B) Though SSU is the only strain to express and produce AexU when grown in DMEM, both SSU and E1 expressed and produced AexU when grown in contact with HeLa cells. As expected from the genomic analyses that demonstrated the absence of AexU from strains E2 and ATCC 7966T, neither of these strains produced AexU under either condition.