FIG 4 .
Overexpression of MAT2 drives shmoo cell formation but does not alter mitochondrial morphology or the mtDNA copy number. (A) The images in the top panel show the round yeast cells of the wild-type (WT) Aa and Aα strains after incubation in V8 medium for 48 h. The images in the bottom panel show the cell morphology (round yeasts and pear-shaped or elongated shmoo cells) of Aa and Aα strains when MAT2 is overexpressed. (B) Quantification of the distribution of yeast cell morphology and shmoo cell morphology in different populations as shown in panel A. (C) Mitotracker CMXRox-labeled wild-type cells and the MAT2oe cells. Stars indicate shmoo cells. (D) The comparison of the COB1/TEF1 ratios between the wild type, the MAT2oe strain, and the mat2Δ mutant cultured in YPD and V8 media. All strains shown here were in the Aα background. Similar results were obtained for strains in the Aa background (see Fig. S3 in the supplemental material).