FIG 2 .

Comparison of proteins identified in SILAC and those with EF-P target motifs. (A) Venn diagram outlining the overlap of: proteins conclusively identified in SILAC, proteins significantly downregulated in WN1308, and proteins containing a PPP, PPG, or APP motif as annotated in the Salmonella serovar Typhimurium strain SL1344 genome (GenBank accession no. FQ312003.1). (B) DAVID analysis of SILAC hits showing a decreased protein level in the ∆efp strain (ratio > 2) and a significance B score of less than 0.01 in at least one trial. Functional annotation clusters showing significant overrepresentation (P value < 0.05) are shown. Cluster breakdown can be found in Table S4 in the supplemental material. (C) DAVID analysis showing the most significantly overrepresented clusters among the 422 proteins that contain an EF-P target motif. For clarity, only groups with P values < 0.001 are shown here. For a full list, see Table S5. (D) DAVID analysis showing the only significantly overrepresented cluster (P value < 0.05) among the 20 proteins that fall into all three categories (contain an EF-P target motif and were significantly downregulated in the ∆efp strain in SILAC). Cluster breakdown can be found in Table S6 in the supplemental material.