Fig. 3.

Photograph of (A) the DeepIsoBUG transfer chamber (1) enabling sediment slurry transfer under pressure (maximum 25 MPa) from low-pressure vessel (2, upside down) to a high-pressure vessel (3, maximum 100 MPa) for inoculation of medium and enrichment. B. Separate low- (4) and high-pressure (5) vessels with ball valves for transfer in and out and gas flushing; low-pressure vessel has large ball valve on top for input of sediment slice(s). Pressure sensor (6) and burst disc (7) also shown. Scale bar on the high-pressure vessel is 22 cm.