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. 2013 May;15(5):472–480. doi: 10.1593/neo.122126

Figure 3.

Figure 3

KLF5 activates JNK signaling in ESCC. (A) By Western blot, phosphorylation of JNK increased five-fold to seven-fold in TE7 and TE15 cells after KLF5 induction for 24 hours, while total JNK was unchanged. (B) Treatment of TE7 and TE15 cells with the small molecule JNK inhibitor SP600125 blocked JNK phosphorylation following KLF5 induction, as indicated by Western blot. (C) When TE7 and TE15 were induced with doxycycline for 24 or 48 hours to express KLF5, treatment with JNK inhibitor inhibited the ability of KLF5 to decrease cell viability, as assessed by MTT assay (*P < .05; **P < .001). (D) Treatment with JNK inhibitor also blocked the proapoptotic effects of KLF5 in TE7 and TE15 cells, as demonstrated by levels of cleaved (cl) caspase 3 and cleaved (cl) PARP. KLF5 was induced for the indicated times.