Skip to main content
. 2013 May;15(5):491–501. doi: 10.1593/neo.13314

Figure 4.

Figure 4

The role of the sarcosine-degrading enzymes, SARDH and PIPOX, in prostate cell lines. (A) FLAG-SARDH and FLAG-PIPOX overexpressing cells showed decreased levels of sarcosine and attenuated invasion in DU145 cells. The invasion was quantitated by absorbance and cells were photographed after invasion through Matrigel and stained with crystal violet (top inset). (B) FLAG-SARDH and FLAG-PIPOX were stably overexpressed in DU145 cells. Cell proliferation assay performed using pooled SARDH, SARDH clone 5, pooled PIPOX, PIPOX clone 2, or vector control cells at the indicated time points showed decreased cell proliferation in SARDH but not PIPOX overexpressing cells. (C) Pooled SARDH or SARDH clone 5 showed decreased number of colonies in soft agar compared to vector control. (D) Same as in B but for shSARDH knockdown RWPE cells (clones 1 and 3) and shPIPOX knockdown RWPE cells (clones 1 and 2) or their respective shNS controls at the indicated time points. (E) Same as in A but for shSARDH and shPIPOX knockdown RWPE cells compared to their respective shNS controls. (F) Same as in C but for shSARDH and shPIPOX knockdown RWPE cells compared to their respective shNS controls.