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. 2013 May;15(5):491–501. doi: 10.1593/neo.13314

Figure 6.

Figure 6

The role of sarcosine pathway enzymes, GNMT and SARDH, in PCa growth in vivo. (A) CAM intravasation assay was performed using shGNMT clone 7, SARDH clone 5, and vector control in DU145 cells. Number of intravasated DU145 cells and tumor weight was decreased in both shGNMT and SARDH overexpressing xenografted cell lines compared to vector control. (B) Liver metastasis in chicken embryos was assessed 8 days following implantation of either shGNMT clone 7 or SARDH clone 5 or vector control cells onto the upper CAM. Total number of metastasized cells were quantified and found to be significantly decreased in both shGNMT and SARDH overexpressing xenografted cell lines compared to vector control. (C) shGNMT (clones 7 and 9) decreased DU145 tumor growth in mice. Means ± SEM are shown, *P < .05. (D) Stably overexpressed SARDH (pool and clone 5) decreased DU145 tumor growth in mice. Means ± SEM are shown, *P < .05. (E) Box plots showing decreased sarcosine levels in shGNMT (clones 7 and 9) mouse xenograft compared to shNS vector control. (F) Box plot showing decreased sarcosine levels in SARDH overexpressed (pool and clone 5) mouse xenograft tumors compared to vector control.