Figure 7.

INZ significantly enhances CIS-induced p53 activation, apoptosis, and tumor suppression. (A) Combination of INZ and CIS markedly suppresses the growth of xenograft tumors derived from H460 cells. Mice bearing H460 xenografts were i.p. treated with INZ (20 mg/kg) everyday or/and CIS (3 mg/kg) once per week or vehicles for 21 days. Images of tumors isolated are presented at the left. The tumor growth is shown by the mean tumor volumes ± SEM (middle), and the final tumor weight is shown in columns (right;n = 6 mice per group; *P < .05). (B) WB analysis of proteins extracted from H460 tumor samples in A with antibodies as indicated on the right. (C) Representative images of TUNEL staining, BrdU, and p53 immunostaining of xenograft tumor sections are presented. Quantification of BrdU and TUNEL staining is expressed as mean ± SEM (n = 4 mice per group).