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. 2013 May 5;368(1617):20110410. doi: 10.1098/rstb.2011.0410

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — Interaction between Ure2p and Ssa1p. (a) Interaction between native Ure2p and ΔA Ssa1p observed by dynamic light scattering measurements. Solid line: Ure2p; dashed-dotted line: ΔA Ssa1p; dashed line: a 1 : 1 mixture of Ure2p and ΔA Ssa1p. (b) Interaction of Ure2p seeds with WT Ssa1p or ΔA Ssa1p observed by the filament pull-down assay as described in the text. The pellets formed after high-speed centrifugation in 40% sucrose were resuspended in buffer and loaded onto a 12% SDS–PAGE gel. Lane 1, low molecular mass marker; lanes 2, 3, 5 and 7: Ure2p seeds incubated alone or with WT Ssa1p, ΔA Ssa1p or BSA, respectively; lanes 4, 6 and 8: WT Ssa1p, ΔA Ssa1p or BSA, respectively, as the molecular weight control.