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. 2013 Jun 6;3(3):20120101. doi: 10.1098/rsfs.2012.0101

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — Membrane topology of pmeLUC. The pmeLUC construct is composed of the full-length coding sequence of luciferase (yellow) inserted in-frame between the N-terminal leader sequence (green) and the C-terminal GPI anchor (violet) of the folate receptor. A c-myc tag (light blue) is also added in-frame for tracking purposes. The pmeLUC protein is targeted and localized to the outer side of the plasma membrane, in close vicinity to all the molecules participating in purinergic signalling (adapted from Pellegatti et al. [24]).