Figure 1.

Overall workflow. (a) An initial set of genes was screened for those loci for which we could perform ASGE measurements and then for which we detected common ASGE within an initial test set of Amboseli individuals. (b) Genes that exhibited common ASGE (n = 34) were subjected to ASGE measurements for all ASGE assay SNP heterozygotes and approximately 7–9 kb of the putative cis-regulatory region was resequenced to identify genetic effects on gene expression (see Table S2 for exact numbers). (c) These genes were then analysed jointly to test for evidence of GEIs involving each environment of interest. The number of genes that survived each progressive screening set is shown in bold at each transition between steps.