Table 2.
Proteins identified in the gel slices of enriched activity from BN-PAGE gels using the reference database of all proteins identified in the ACT-3 metagenome (only the top seven hits were listed for each gel slice). Bold indicates reductive dehalogenases.
| culture | BN-PAGE slice | IMG gene locus tag | peptide hits | annotation | putative organism |
|---|---|---|---|---|---|
| CF subculture | BP-3 | DHTCA2_00302240 | 25 | OAH/OAS sulfhydrylase | Dehalobacter |
| DHTCA2_00199880 | 20 | chaperone protein DnaK | Dehalobacter | ||
| DHTCA2_00295340 | 14 | chaperonin GroEL | Dehalobacter | ||
| DHTCA2_00242140 | 13 | citrate lyase beta subunit | Dehalobacter | ||
| DHTCA2_00272470 | 10 | dihydroxy-acid dehydratase | Dehalobacter | ||
| DHTCA2_00327390 | 8 | reductive dehalogenase CfrA fragment | Dehalobacter | ||
| DHTCA2_00269400 | 7 | formate dehydrogenase alpha subunit | Dehalobacter | ||
| BP-7 | DHTCA2_00197470 | 23 | reductive dehalogenase CfrA fragment | Dehalobacter | |
| DHTCA2_00327390 | 18 | reductive dehalogenase CfrA fragment | Dehalobacter | ||
| DHTCA2_00217090 | 12 | uroporphyrin-III C-methyltransferase | Dehalobacter | ||
| DHTCA2_00199880 | 7 | chaperone protein DnaK | Dehalobacter | ||
| DHTCA2_00306260 | 7 | Hup-type Ni,Fe-hydrogenase large subunit | Dehalobacter | ||
| DHTCA2_00474830 | 5 | alcohol dehydrogenase, class IV | Desulfovibrio | ||
| DHTCA2_00941900 | 5 | translation elongation factor TU | Desulfovibrio | ||
| DCA subculture | BP-2 | DHTCA2_00532520 | 27 | carbon monoxide dehydrogenase large subunit | Desulfovibrio |
| DHTCA2_00486690 | 11 | methanol–cobalamin methyltransferase subunit B | Methanosarcina | ||
| DHTCA2_00114900 | 10 | carbon monoxide dehydrogenase large subunit | Desulfovibrio | ||
| DHTCA2_00239010 | 10 | formyltetrahydrofolate synthetase | Dehalobacter | ||
| DHTCA2_00199880 | 9 | chaperone protein DnaK | Dehalobacter | ||
| DHTCA2_00269400 | 8 | formate dehydrogenase alpha subunit | Dehalobacter | ||
| DHTCA2_00474830 | 5 | alcohol dehydrogenase, class IV | Desulfovibrio | ||
| BP-6 | DHTCA2_00474830 | 17 | alcohol dehydrogenase, class IV | Desulfovibrio | |
| DHTCA2_00199880 | 14 | chaperone protein DnaK | Dehalobacter | ||
| DHTCA2_00502190 | 9 | alcohol dehydrogenase, class IV | Desulfovibrio | ||
| DHTCA2_00603650 | 6 | reductive dehalogenase DcrA fragment | Dehalobacter | ||
| DHTCA2_00306260 | 5 | Hup-type Ni,Fe-hydrogenase large subunit | Dehalobacter | ||
| DHTCA2_00201920 | 4 | branched-chain amino acid transporter | Dehalobacter | ||
| DHTCA2_00609600 | 4 | pyridoxamine 5’-phosphate oxidase | Dehalobacter | ||
| ACT-3 parent culture | BP-4 | DHTCA2_00202930 | 9 | putative cell wall-binding domain | Dehalobacter |
| DHTCA2_00199880 | 8 | chaperone protein DnaK | Dehalobacter | ||
| DHTCA2_00269400 | 5 | formate dehydrogenase alpha subunit | Dehalobacter | ||
| DHTCA2_00755650 | 4 | DNA polymerase sliding clamp subunit | Methanohalobium | ||
| DHTCA2_00474830 | 3 | alcohol dehydrogenase, class IV | Desulfovibrio | ||
| DHTCA2_00941900 | 3 | translation elongation factor TU | Desulfovibrio | ||
| DHTCA2_00331370 | 3 | acyl CoA:acetate/3-ketoacid CoA transferase | Clostridium | ||
| BP-7 | DHTCA2_00637940 | 11 | methanol–cobalamin methyltransferase subunit B | Methanohalophilus | |
| DHTCA2_01115960 | 11 | methanol–cobalamin methyltransferase subunit B | Methanosalsum | ||
| DHTCA2_00197470 | 10 | reductive dehalogenase CfrA fragment | Dehalobacter | ||
| DHTCA2_00474830 | 9 | alcohol dehydrogenase, class IV | Desulfovibrio | ||
| DHTCA2_00941900 | 8 | translation elongation factor TU | Desulfovibrio | ||
| DHTCA2_00327390 | 7 | reductive dehalogenase CfrA fragment | Dehalobacter | ||
| DHTCA2_00306770 | 6 | translation elongation factor TU | Dehalobacter |